Eastern Hemlock Research

at Holden Forests & Gardens

Researchers at Holden Forests & Gardens are working to save eastern hemlock trees from invasive pests through resistance breeding and innovative research.


The eastern hemlock (Tsuga canadensis) plays a vital role in our local forest ecosystems. But invasive forest pests and diseases, including hemlock woolly adelgid (Adelges tsugae) and elongate hemlock scale (Fiorinia externa), threaten these trees’ survival. In some areas, these tiny invaders have already killed nearly 80 percent of hemlocks.

Better Techniques for HWA-Resistance Breeding


One major goal of the hemlock team at Holden is to develop better tools for studying and breeding resistant trees. They’re creating a new screening method to measure how well individual trees can fight off hemlock woolly adelgid (HWA), and crucially, whether that resistance comes from heritable genetic traits that can be passed down through breeding. The team is also experimenting with techniques to make trees flower and produce cones much earlier than normal — at just 2-5 years old instead of the typical 30 years. Speeding up this timeline will allow researchers to breed multiple generations much faster.

This work is in partnership with The Great Lakes Basin Forest Health Collaborative, U.S. Forest Service Northern Research Station, The Nature Conservancy’s Trees in Peril, and the New Jersey Forest Service, with additional funding from the Pennsylvania Department of Conservation and Natural Resources.

To study the effects of these pests on the trees, and potential mechanisms for resistance, it’s helpful for researchers to have access to hemlock trees from a number of different locations that they can study across their entire lives. At Holden, researchers grew a cohort of trees in 2021 that will provide research opportunities for years to come. (Credit: Rachel Kappler/HF&G)

Breeding Resistant Hemlocks


Holden Arboretum is putting these methods into action through an eastern hemlock resistance breeding program. The team and their collaborators have identified promising candidates from across the eastern United States: “lingering hemlocks” that survived the initial wave of HWA infestation and may carry genetic resistance. Researchers are creating clones of these trees through cuttings, testing their resistance using the new screening methods, and establishing them in greenhouse conditions designed to accelerate cone and pollen production. This breeding population will become the foundation for developing the next generation of HWA-resistant hemlocks.

Left, Holden volunteers transplant 5-year-old hemlocks to larger pots that were grown at Holden from local seeds. Right, hemlock seedlings grown by New Jersey Forest Service and sent to Holden for research as a part of this collaboration. (Credit: Rachel Kappler/HF&G)

Effects of Pesticides on Hemlock Health


Pesticides can kill hemlock woolly adelgid — but what do they do to the trees themselves? Researchers in Holden’s soil ecology lab are investigating that question by applying Dinotefuran, a commonly used pesticide, to hemlock trees. Early results show that the treatment may come with tradeoffs: The bacteria living on hemlock leaves changed dramatically with pesticide application. These findings suggest that while pesticides can eliminate the pest, they might also weaken trees’ ability to grow and fight off other diseases by disrupting the microscopic organisms that help keep them healthy.

Left, studying the effects of hemlock woolly adelgid on the microbiome. Right, eastern hemlock infested with elongate scale. (Credit: David Burke/HF&G)

From the Get Growing Blog


For a deeper dive into our hemlock research, explore these recent blog posts from Holden scientists and science writers:

Know the Forest Pests That Live in Your Area: Holden Researchers Get the Word Out

by Anna Funk | Nov 20, 2025

Do you know what the symptoms of beech leaf disease look like? What about hemlock woolly adelgid — can you tell it from elongate hemlock scale, and did you know these are both insects that can harm trees? If your answers were “no, no, and no,” you’re not alone. Plenty of… Keep reading.

Protecting Eastern Hemlocks from Tiny Tree-Killing Invaders

by Albert Jackson | Jan 9, 2024

The Holden Conservation Department is on the hunt for tiny tree-killing pests. During the early and mid-20th centuries, two non-native tree dwelling insects were unintentionally introduced to the eastern United States through the sale of infected East Asian trees. Today, these two insects have spread through most eastern hemlock forests… Keep reading.

Monitoring Hemlock Woolly Adelgid Colonization

by Danny Dlugos | Mar 13, 2024

Eastern hemlock (Tsuga canadensis) is one of the most recognized trees in Northeast Ohio. Native to the eastern United States and Canada, it ranges from Nova Scotia south along the Appalachian Mountains to Alabama, and slightly westward throughout the Great Lakes region. In our area, it is often seen along… Keep reading.

Meet our Hemlock Experts


Danny Dlugos
Danny Dlugos Senior Research Specialist
Dr. Dlugos is an expert in the management of plant diseases and is trained in disease diagnostics. His interests and experiences span plant topics of regulatory significance including plant diseases, plant pests, and invasive plant species, as well as education and public outreach related to these issues. Before coming to Holden, he worked in a cooperative extension lab, was a visiting assistant professor at Miami University, and was lecturer at John Carroll University.
Rachel Kappler
Rachel Kappler Postdoctoral Research Associate
Dr. Kappler is a conservation ecologist interested in species protection, particularly within the forest ecosystem. Currently, her work involves eastern hemlock, American beech and ash species. She is collaborating with partners to work towards a future tree breeding program that can reintroduce trees with increased defense against invasive pests and diseases. This involves research quantifying individual tree defenses towards invasive pest and disease species. Previously, she helped facilitate stakeholders’ activities related to resistance breeding in trees. She has also researched ecological interactions and restoration efforts within ash forests, especially related to the impact of emerald ash borer on natural remnant populations of green ash trees.
David Burke
David Burke Vice President for Science and Conservation
Dr. Burke is a soil and microbial ecologist whose work spans the gamut from soil fungi to forest pathology. He is a leader in the study of beech leaf disease, identifying how the disease is spread and understanding the mechanisms of the disease. Other research efforts in forest pathology include studies on hemlock trees and the invasive pest hemlock wooly adelgid. Dr. Burke’s soil ecology research focuses on the interactions between plants and the microorganisms that live in the soil, like the mycorrhizal fungi that form mutually beneficial relationships with plants.
Rory Schiafo
Rory Schiafo Postdoctoral Research Associate
Rory Schiafo is a plant community ecologist whose research focuses on forest ecology, restoration, and conservation. She is currently studying the conservation of eastern hemlock, working to identify populations that may show resistance to the invasive forest pest, hemlock woolly adelgid. By pinpointing potentially resistant trees, her research aims to inform seed collection and breeding programs designed to increase and spread genetic resistance across the species’ range. In addition, Rory collaborates with federal, nonprofit, academic, and private partners to analyze long-term forest monitoring datasets from across the eastern United States. This work seeks to better understand patterns of hemlock mortality and forest change in the face of adelgid invasion. Rory is completing her PhD through Northwestern University and the Chicago Botanic Garden, where her doctoral research examines plant diversity and restoration dynamics in Midwestern oak savannas.

About the Long Center for Plant & Environmental Science


At the Holden Arboretum, the scientists at the Ellen Corning Long and T. Dixon Long Center for Plant and Environmental Science take pride in pioneering the science of plants and trees, conducting nationally recognized research while fostering the next generation of leaders in this space. Learn more about the Long Science Center ▶

About Holden Forests & Gardens


Holden Forests & Gardens is made up of two of Northeast Ohio’s most important environmental and cultural institutions — the Holden Arboretum and Cleveland Botanical Garden — and is the 12th largest public garden in the country. HF&G is a leader in plant science, advancing groundbreaking research and sharing discoveries that protect forests and natural areas. Learn more about Holden Forests & Gardens ▶

Keep in Touch


Stay up to date on our latest hemlock discoveries: Subscribe to The Lab & Field, Holden Forests & Gardens’ science and conservation newsletter.

Contact Holden Forests & Gardens

Media please contact:
Stacey Vaselaney
slvaselaney@slvpr.com
(216)905-0908

General inquiries:
Anna Funk
Science Communication Specialist
afunk@holdenfg.org

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