Sowing Orchid Seeds In Vitro

Fri., Dec. 11, 2020

By Connor Ryan, MS, Rhododendron Collections Manager

Orchids are among the most ornamental and diverse groups of plants in the world. We often think of them as epiphytes, growing on trees in the tropics. Many would be surprised to know that there are orchids native to Ohio and that they do not grow in trees. Our native orchids grow in the ground and are called terrestrial orchids. Ohio has approximately 47 native orchid species, and of them, the lady’s slippers may be the showiest.  

Ohio has 5 native species of lady’s slippers (genus Cypripedium) and one naturally occurring hybrid. Holden has three of these species growing in our natural areas. Lady’s slippers are characterized by a modified sepal, or labellum, that is shaped, you guessed it, like a lady’s slipper. This labellum serves to attract pollinators, mainly bees, to the orchid. Once a lady’s slipper is pollinated, a fruit called a capsule forms and maturity splits open to disperse thousands of tiny, dust-like seeds.

Three capsules (left) and the thousands of seeds contained within (right).

Orchids usually require a special relationship with fungi to germinate and grow successfully. Consequently, they can be tricky for gardeners and horticulturists to grow from seed if you do not have the appropriate fungi present. These fungi, termed mycorrhizal fungi, infect recently germinated seedlings and transport essential nutrients to the growing plant.  Despite this apparent need for a fungal partner to be able to grow orchids successfully from seeds, scientists and orchid enthusiasts have figured out a way to bypass this need – in vitro seed germination. 

Cypripedium seeds can be sown in vitro – in a sterile, laboratory environment on a nutrient agar, or gel – to supply the essential nutrients that they would otherwise get from a mycorrhizal fungus. In partnership with folks in the Horticulture Department, we visited one of Holden’s natural areas in October to collect seeds of the state threatened Cypripedium reginae, the showy lady’s slipper. This week, we sowed these seeds in test tubes on nutrient agar. Now they are sitting in a box in the dark and will remain there for at least month when we will check for germination. Fingers crossed for good germination!

Orchid seeds in vitro

I hope to provide periodic updates on this blog as we move along in the orchid germination process. And if things go well, we’ll try a few more species next year. The resulting orchid seedlings can be used for bolstering the current population, for backing them up in the Holden plant collections, and for educating our visitors about biodiversity and conservation.

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Connor Ryan, MS

Connor Ryan, MS

Rhododendron Collections Manager

As the Rhododendron Collections Manager at Holden Forests & Gardens (HF&G), my job is to curate and build the Rhododendron collections across all three HF&G campuses. The genus Rhododendron, which consists of roughly 1,000 total species, contains four groups of plants significant to Northeast Ohio: evergreen azaleas, deciduous azaleas, large leaf rhododendrons and small leaf rhododendrons. This diversity, coupled with the ability of species to freely hybridize within their respective groups, provides ample opportunities to collect and display an array of interesting and rare rhododendrons from around the world. During the growing season, I am most frequently found at the David G. Leach Research Station, a 30-acre facility dedicated to Rhododendron breeding, evaluation, and display in Madison, Ohio. I am actively involved in the Rhododendron breeding program at HFG started by David G. Leach, PhD, and continued by Steve Krebs, PhD. I am interested in ex-situ plant conservation, ornamental plant breeding and evaluation, and plant propagation. Feel free to reach out if you are interested in collaborating or if you want to talk plants.

Learn more about me


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